Matrices listed below were spiked with certain level of CYP1A2 and the recovery rates were calculated by comparing the measured value to the expected amount of CYP1A2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-98
92
EDTA plasma(n=5)
88-102
94
heparin plasma(n=5)
89-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYP1A2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CYP1A2 ELISA Kit allows for the in vitro quantitative determination of CYP1A2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CYP1A2(Cytochrome P450, family 1, subfamily A, polypeptide 2) ELISA Kit