Human Cyclin-dependent Kinase 20

Mfr.No.

E3894Hu

Description

This sandwich kit is for the accurate quantitative detection of human Cyclin-dependent Kinase 20 (also known as CCRK) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CAK-kinase p42;CDK 20;CDK20;CDK-activating kinase p42;Cell cycle-related kinase;Cell division protein kinase 20;Cyclin-dependent kinase 20;Cyclin-dependent protein kinase H;Cyclin-kinase-activating kinase p42;

Reactivity

Human

Detection Range

20-7000ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

38695

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

10.25ng/ml

Background

Required for high-level Shh responses in the developing neural tube. Together with TBC1D32, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling (By similarity). Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160'.

Gene ID	23552
UniProt ID	Q8IZL9
Antigen	CDK20

Research Area

Others

Protocol

These standard curves of E3894Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000ng/ml	2.092	1.998	1.962
	1.904
2000ng/ml	1.056	1.068	1.032
	1.08
1000ng/ml	0.533	0.496	0.46
	0.458
500ng/ml	0.291	0.284	0.248
	0.276
250ng/ml	0.132	0.143	0.107
	0.154
0ng/ml	0.041	0.036	0
	0.03

Precision

We measured random samples of E3894Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2922.9	89.18	3.1
Intra-Assay	2	18	1139.6	57.37	5
Intra-Assay	3	18	1602.1	63.33	4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105332	E3894Hu	48T	$320		Add to Cart
BHE12105332	E3894Hu	96T	$458		Add to Cart

Human Cyclin-dependent Kinase 20 - CCRK ELISA Kit

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