Human CXCL13 / BLC / BCA1 ELISA Kit PicoKine®

Mfr.No.

EK0739

Sensitivity

<1 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.026	0.053	0.118	0.159	0.287	0.510	1.151	1.889

Description

Human CXCL13 / BLC / BCA1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CXCL13 in cell culture supeatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-X-C motif chemokine 13;Angie;B cell-attracting chemokine 1;BCA-1;B lymphocyte chemoattractant;CXC chemokine BLC;Small-inducible cytokine B13;CXCL13;BCA1, BLC, SCYB13;

Molecular Weight

12664 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V23-R94

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CXCL13

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma, Saliva

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	32	216	441	30	232	416
Standard deviation	2.2	16.84	24.69	2.19	20.64	27.3%8
CV (%)	6.9%	7.8%	5.6%	7.3%	8.9%	6.8%

Protein Function

Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B- lymphocytes. Binds to BLR1/CXCR5.

Background

C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	32	31	36	31	32	2.06	6.4%
Sample 2	216	244	229	223	228	10.31	4.5%
Sample 3	441	424	416	416	424	10.2	2.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	10563
Gene Name	CXCL13
UniProt ID	O43927
Protein Name	C-X-C motif chemokine 13
Tissue Specificity	Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.
Sequence	Belongs to the intercrine alpha (chemokine CxC) family.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000596	EK0739	96 wells/kit	$499		Add to Cart

Human CXCL13 / BLC / BCA1 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human CXCL13 / BLC / BCA1 ELISA Kit PicoKine®

Rate it now..!!