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Home / ELISA Kit

Human CUGBP Elav-like family member 3 (TNRC4) ELISA Kit

SKU : BHE13702397

CELF proteins, including CELF3, bound RNA containing muscle-specific splicing enhancer (MSE) elements and could activate MSE-dependent exon inclusion of cardiac troponin T (TNNT2) minigenes in transfected quail fibroblasts.The deduced 462-amino acid protein contains 2 N-terminal RNA recognition motifs (RRMs), a C-terminal RRM, several potential phosphorylation sites, and a nuclear localization signal near the C terminus. activated inclusion of exon 10 in a tau (MAPT) minigene. Deletion and mutation analyses showed that the activity of TNRC4 on tau exon 10 splicing required RRM2 in TNRC4 and an intronic element in tau pre-mRNA. The polyglutamine sequence of TNRC4 had no effect on tau exon 10 splicing. Tnrc4 -/- mice exhibited decreased inclusion of exon 10 in tau mRNA.

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Mfr.No.	KTE60178
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human CUGBP Elav-like family member 3 (TNRC4) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC4.
Alternative Names	TNRC4; BRUNOL1; CAGH4; CELF3; ERDA4; MGC57297; CAG repeat domain; CUG-BP and ETR-3 like factor 3; expanded repeat domain; CAG/CTG 4
Detection Method	Colorimetric
Kit Components	Human CUGBP Elav-like family member 3 microplate Human CUGBP Elav-like family member 3 standard Human CUGBP Elav-like family member 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human CUGBP Elav-like family member 3 (TNRC4) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNRC4. No significant cross-reactivity or interference between Human TNRC4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	CELF proteins, including CELF3, bound RNA containing muscle-specific splicing enhancer (MSE) elements and could activate MSE-dependent exon inclusion of cardiac troponin T (TNNT2) minigenes in transfected quail fibroblasts.The deduced 462-amino acid protein contains 2 N-terminal RNA recognition motifs (RRMs), a C-terminal RRM, several potential phosphorylation sites, and a nuclear localization signal near the C terminus. activated inclusion of exon 10 in a tau (MAPT) minigene. Deletion and mutation analyses showed that the activity of TNRC4 on tau exon 10 splicing required RRM2 in TNRC4 and an intronic element in tau pre-mRNA. The polyglutamine sequence of TNRC4 had no effect on tau exon 10 splicing. Tnrc4 -/- mice exhibited decreased inclusion of exon 10 in tau mRNA.
Notes	This Human CUGBP Elav-like family member 3 (TNRC4) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC4 in samples. An antibody specific for TNRC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNRC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNRC4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNRC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	11189

Applications	ELISA
Applications Notes	This Human CUGBP Elav-like family member 3 (TNRC4) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC4 in samples. An antibody specific for TNRC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNRC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNRC4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNRC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702397	KTE60178	48 T	$339		Add to Cart
BHE13702397	KTE60178	96 T	$589		Add to Cart
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BHE13702397	KTE60178	96 Tx50	$24569		Add to Cart

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