Human CTRP9 ELISA Kit PicoKine®

Mfr.No.

EK1881

Sensitivity

<10 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	46.9	93.7	187.5	375	750	1500	3000
O.D.	0.007	0.026	0.069	0.162	0.354	0.799	1.518	2.021

Description

Human CTRP9 ELISA Kit PicoKine® (96 Tests). Quantitate Human C1QTNF9 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Complement C1q and tumor necrosis factor-related protein 9A; Complement C1q and tumor necrosis factor-related protein 9; C1QTNF9; C1QTNF9A; UNQ6503; PRO21380

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q20-P333

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CTRP9

Subcellular Localization

Secreted

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	65	280	1368	73	291	1425
Standard deviation	3.19	14.28	79.34	5.18	22.12	111.15
CV (%)	4.9%	5.1%	5.8%	7.1%	7.6%	7.8%

Protein Function

Probable adipokine. Activates AMPK, AKT, and p44/42 MAPK signaling pathways.

Background

The C1QTNF9 gene is conserved in chimpanzee, Rhesus monkey, and zebrafish. This gene is mapped to 13q12.12. 42 organisms have orthologs with human gene C1QTNF9.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	65	59	68	62	63	3.35	5.3%
Sample 2	280	327	277	285	292	20.26	6.9%
Sample 3	1368	1367	1278	1292	1326	41.54	3.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	338872
Gene Name	C1QTNF9
UniProt ID	P0C862
Tissue Specificity	Expressed predominantly in adipose tissue.

Applications	ELISA
Research Area	Cytokines,Immunology,Innate Immunity,Secreted Molecules,TNF Superfamily

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001427	EK1881	96 wells/kit	$499		Add to Cart

Human CTRP9 ELISA Kit PicoKine®

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