Human CTRC/Chymotrypsin-C ELISA Kit PicoKine®

Mfr.No.

EK2001

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.024	0.152	0.244	0.463	0.746	1.212	1.795	2.321

Description

Human CTRC ELISA Kit PicoKine® (96 Tests). Quantitate Human CTRC in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Chymotrypsin-C; Caldecrin; CTRC; CLCR

Immunogen

Expression system for standard: CHO; Immunogen sequence: V30-L268

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CTRC

Subcellular Localization

Extracellular region.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	229	1188	4782	314	1249	4914
Standard deviation	12.14	66.53	277.36	20.72	86.18	358.72
CV (%)	5.3%	5.6%	5.8%	6.6%	6.9%	7.3%

Protein Function

Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity.

Background

Chymotrypsin C (CTRC), also known as caldecrin, is a serine protease and a member of the peptidase S1 family. This gene is mapped to 1p36.21. This gene encodes a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alteatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	229	278	272	238	254	21.09	8.3%
Sample 2	1188	1214	1275	1228	1226	31.59	2.5%
Sample 3	4782	5075	5245	4924	5006	172.32	3.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	11330
Gene Name	CTRC
UniProt ID	Q99895
Protein Name	Chymotrypsin-C
Tissue Specificity	Pancreas.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001538	EK2001	96 wells/kit	$750		Add to Cart

Human CTRC/Chymotrypsin-C ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human CTRC/Chymotrypsin-C ELISA Kit PicoKine®

Rate it now..!!