Human CTRC/Chymotrypsin-C ELISA Kit PicoKine®

Mfr.No.

EK2001

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.024	0.152	0.244	0.463	0.746	1.212	1.795	2.321

Description

Human CTRC ELISA Kit PicoKine® (96 Tests). Quantitate Human CTRC in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Chymotrypsin-C; Caldecrin; CTRC; CLCR

Immunogen

Expression system for standard: CHO; Immunogen sequence: V30-L268

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CTRC

Subcellular Localization

Extracellular region.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	229	1188	4782	314	1249	4914
Standard deviation	12.14	66.53	277.36	20.72	86.18	358.72
CV (%)	5.3%	5.6%	5.8%	6.6%	6.9%	7.3%

Protein Function

Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity.

Background

Chymotrypsin C (CTRC), also known as caldecrin, is a serine protease and a member of the peptidase S1 family. This gene is mapped to 1p36.21. This gene encodes a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	229	278	272	238	254	21.09	8.3%
Sample 2	1188	1214	1275	1228	1226	31.59	2.5%
Sample 3	4782	5075	5245	4924	5006	172.32	3.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	11330
Antigen	CTRC/Chymotrypsin-C
Gene Name	CTRC
UniProt ID	Q99895
Protein Name	Chymotrypsin-C
Tissue Specificity	Pancreas.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001538	EK2001	96 wells/kit	$750		Add to Cart

Human CTRC/Chymotrypsin-C ELISA Kit PicoKine®

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