Human CRP ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ1316

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.005	0.046	0.1	0.174	0.448	0.83	1.374	1.959

Description

Human CRP ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CRP in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA).

Alternative Names

C reactive protein pentraxin related; C-reactive protein; C-reactive protein (1-205); CRP; CRP_HUMAN; MGC149895; MGC88244; Pentraxin 1; Pentraxin 1 short; PTX 1; PTX1;

Immunogen

Expression system for standard: NS0; Immunogen sequence: F17-P224

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CRP

Subcellular Localization

Secreted

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	593	1488	8142	565	1551	8792
Standard deviation	36.17	62.49	643.21	38.42	75.99	861.61
CV (%)	6.1%	4.2%	7.9%	6.8%	4.9%	9.8%

Protein Function

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Background

C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	1401
Gene Name	CRP
UniProt ID	P02741
Tissue Specificity	Found in plasma.

Applications	ELISA
Research Area	Apoptosis,Atherosclerosis,Cardiovascular,Chromatin Binding Proteins,Complement,DNA/RNA Binding,Epigenetics and Nuclear Signaling,Immunology,Innate Immunity,Macrophage/Inflammation,Metabolism,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000104	EZ1316	5 plates/kit	$500		Add to Cart

Human CRP ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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