Human CRISP3 ELISA Kit PicoKine®

Mfr.No.

EK1284

Sensitivity

<50 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.026	0.911	1.198	1.383	1.738	1.844	1.976	2.177

Description

Human CRISP3 ELISA Kit PicoKine® (96 Tests). Quantitate Human CRISP3 in cell culture supeatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cysteine-rich secretory protein 3;CRISP-3;Specific granule protein of 28 kDa;SGP28;CRISP3;

Molecular Weight

27630 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: N21-Y245

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CRISP3

Subcellular Localization

Secreted. In neutrophils, localized in specific granules.

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	110	383	1477	119	408	1764
Standard deviation	4.51	23.75	66.47	5.59	26.11	95.26
CV (%)	4.1%	6.2%	4.5%	4.7%	6.4%	5.4%

Background

Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	110	113	130	111	116	8.15	7.0%
Sample 2	383	419	441	377	405	26.26	6.4%
Sample 3	1477	1600	1623	1582	1570	55.90	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	10321
Gene Name	CRISP3
UniProt ID	P54108
Protein Name	Cysteine-rich secretory protein 3
Tissue Specificity	Salivary gland, pancreas and prostate > epididymis, ovary, thymus and colon.
Sequence	Belongs to the CRISP family.

Applications	ELISA
Research Area	Cancer,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000873	EK1284	96 wells/kit	$499		Add to Cart

Human CRISP3 ELISA Kit PicoKine®

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