Human CRELD2 ELISA Kit PicoKine®

Mfr.No.

EK1983

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	165	312	625	1250	2500	5000	10000
O.D.	0.052	0.109	0.141	0.236	0.399	0.791	1.318	2.094

Description

Human CRELD2 ELISA Kit PicoKine® (96 Tests). Quantitate Human CRELD2 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cysteine-rich with EGF-like domain protein 2; CRELD2; UNQ185/PRO211

Immunogen

Expression system for standard: NS0; Immunogen sequence: A25-L353

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CRELD2

Subcellular Localization

Secreted. Endoplasmic reticulum.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	153	696	3699	209	874	4132
Standard deviation	6.43	42.46	240.44	11.29	37.58	231.39
CV (%)	4.2%	6.1%	6.5%	5.4%	4.3%	5.6%

Protein Function

May regulate transport of alpha4-beta2 neuronal acetylcholine receptor.

Background

Cysteine-rich with EGF-like domain protein 2 is a protein that in humans is encoded by the CRELD2 gene. It is mapped to 22q13.33. CRELD2 was identified by searching a sequence database using CRELD1 as the query. Like CRELD1, with which it shares 38% amino acid identity, the deduced 321-amino acid CRELD2 protein contains EGF-like repeats and a highly conserved domain rich in glutamic acid and tryptophan (WE domain). The major distinction between CRELD1 and CRELD2 is that CRELD1 has 2 type III transmembrane domains in its C terminus, while CRELD2 has none. CRELD2 shares 67% and 69% amino acid identity with its Chinese hamster and mouse homologs, respectively.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	153	154	147	158	153	3.93	2.5%
Sample 2	696	700	750	711	714	21.35	2.9%
Sample 3	3699	3606	3621	3798	3681	76.22	2.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	79174
Gene Name	CRELD2
UniProt ID	Q6UXH1
Tissue Specificity	Ubiquitously expressed.

Applications	ELISA
Research Area	Cell Biology,Ligand-Gated Ion Channels,Neuroscience,Neurotransmission,Receptors / Channels

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001488	EK1983	96 wells/kit	$499		Add to Cart

Human CRELD2 ELISA Kit PicoKine®

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