Human Creatine Kinase S-type - Mitochondrial

Mfr.No.

E5124Hu

Sensitivity

0.14mU/mL

Detection Range

0.3-90mU/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Basic-type mitochondrial creatine kinase;CKMT2;Creatine kinase S-type, mitochondrial;Mib-CK;Sarcomeric mitochondrial creatine kinase;SMTCK;S-MtCK

Molecular Weight

47504

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E5124Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	18.84	1.06	5.64
Intra-Assay	2	18	31.45	1.2	3.81
Intra-Assay	3	18	5.06	0.27	5.29

Background

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Protocol

These standard curves of E5124Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48mU/mL	2.078	2.224	2.187
	2.37
24mU/mL	1.256	1.292	1.255
	1.328
12mU/mL	0.696	0.775	0.738
	0.854
6mU/mL	0.435	0.456	0.419
	0.478
3mU/mL	0.179	0.184	0.147
	0.19
0mU/mL	0.042	0.037	0
	0.032

Gene ID	1160
UniProt ID	P17540

Research Area	Tags & Cell Markers

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106487	E5124Hu	48T	$320		Add to Cart
BHE12106487	E5124Hu	96T	$458		Add to Cart

Human Creatine Kinase S-type - Mitochondrial - CKMT2 ELISA Kit

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