Matrices listed below were spiked with certain level of COTL1 and the recovery rates were calculated by comparing the measured value to the expected amount of COTL1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-101
94
EDTA plasma(n=5)
85-105
95
heparin plasma(n=5)
86-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COTL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
COTL1 ELISA Kit allows for the in vitro quantitative determination of COTL1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human COTL1(Coactosin-like protein) ELISA Kit