Human Corticosteroid 11-beta-dehydrogenase Isozyme 2

Mfr.No.

E6455Hu

Sensitivity

0.024ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Corticosteroid 11-beta-dehydrogenase Isozyme 2 (also known as HSD11B2) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

11-beta-HSD;11-beta-HSD type II;11-beta-HSD2;11-beta-hydroxysteroid dehydrogenase type 2;11-beta-hydroxysteroid dehydrogenase type II;11-DH2;11-HSD type II;Corticosteroid 11-beta-dehydrogenase isozyme 2;HSD11B2;NAD-dependent 11-beta-hydroxysteroid dehydrogenase;Short chain dehydrogenase/reductase family 9C member 3

Molecular Weight

44127

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E6455Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.4	0.11	4.4
Intra-Assay	2	18	1.7	0.09	5.4
Intra-Assay	3	18	0.9	0.05	6.1

Background

Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.

Protocol

These standard curves of E6455Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.281	2.163	2.108
	2.044
4ng/ml	1.805	1.808	1.753
	1.811
2ng/ml	1.182	1.171	1.116
	1.159
1ng/ml	0.724	0.728	0.673
	0.732
0.5ng/ml	0.355	0.359	0.304
	0.363
0ng/ml	0.054	0.055	0
	0.055

Gene ID	3291
UniProt ID	P80365

Research Area	Signal Transduction; Cell Biology; Cardiovascular; Cancer; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107817	E6455Hu	48T	$320		Add to Cart
BHE12107817	E6455Hu	96T	$458		Add to Cart

Human Corticosteroid 11-beta-dehydrogenase Isozyme 2 - HSD11B2 ELISA Kit

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