Human Complement Factor H/CFH ELISA Kit PicoKine®

Mfr.No.

EK1399

Sensitivity

<50 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.017	0.135	0.233	0.445	0.857	1.343	1.813	2.017

Description

Human Complement Factor H/CFH ELISA Kit PicoKine® (96 Tests). Quantitate Human CFH in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Complement factor H;H factor 1;CFH;HF, HF1, HF2;

Molecular Weight

139096 MW

Immunogen

Target protein isolated from human plasma

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CFH

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	3203	13518	53844	3406	12935	57149
Standard deviation	233.81	784.04	3822.92	303.13	905.45	4971.96
CV (%)	7.3%	5.8%	7.1%	8.9%	7%	8.7%

Protein Function

Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alteative complement pathway.

Background

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alteativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alteative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	3203	2994	3170	3050	3104	85.4	2.7%
Sample 2	13518	12944	13467	11714	12910	726.54	5.6%
Sample 3	53844	53833	53094	52680	53362	497.77	0.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3075
Gene Name	CFH
UniProt ID	P08603
Protein Name	Complement factor H
Tissue Specificity	Expressed by the liver and secreted in plasma.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000996	EK1399	96 wells/kit	$499		Add to Cart

Human Complement Factor H/CFH ELISA Kit PicoKine®

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