Human Complement Component C8 Gamma Chain

Mfr.No.

E5113Hu

Sensitivity

0.25ng/ml

Detection Range

0.5-150ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

C8G;Complement component C8 gamma chain

Molecular Weight

22277

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5113Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	42	1.89	4.5
Intra-Assay	2	18	29.5	1.38	4.7
Intra-Assay	3	18	16.7	0.88	5.3

Background

C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol.

Protocol

These standard curves of E5113Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/ml	1.962	2.127	2.087
	2.292
40ng/ml	1.349	1.432	1.392
	1.516
20ng/ml	0.54	0.656	0.615
	0.771
10ng/ml	0.358	0.408	0.368
	0.459
5ng/ml	0.167	0.171	0.131
	0.175
0ng/ml	0.029	0.04	0
	0.051

Gene ID	733
UniProt ID	P07360

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106476	E5113Hu	48T	$320		Add to Cart
BHE12106476	E5113Hu	96T	$458		Add to Cart

Human Complement Component C8 Gamma Chain - C8G ELISA Kit

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