Human Complement C1R ELISA Kit PicoKine®

Mfr.No.

EK1954

Sensitivity

<0.1 ng/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.051	0.096	0.127	0.207	0.363	0.646	1.115	1.716

Description

Human Complement C1R ELISA Kit PicoKine® (96 Tests). Quantitate Human C1R in cell culture supeatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Complement C1r subcomponent; Complement component 1 subcomponent r; C1R

Immunogen

Expression system for standard: NS0; Immunogen sequence: S18-D705

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human C1R

Subcellular Localization

Secreted

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2705	11224	42475	3058	11987	42819
Standard deviation	129.84	583.65	1953.85	186.54	707.23	2740.42
CV (%)	4.8%	5.2%	4.6%	6.1%	5.9%	6.4%

Protein Function

C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.

Background

Complement C1r subcomponent is a protein involved in the complement system of the innate immune system. It is mapped to 12p13.31. In humans, C1r is encoded by the C1R gene. C1r along with C1q and C1s form the C1 complex, which is the first component of the serum complement system. C1r is an enzyme that activates C1s to its active form, by proteolytic cleavage.C1r has been shown to interact with C1s. C1r cleaves C1s to form the active form of C1s.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	2705	2824	2677	2767	2743	56.86	2.0%
Sample 2	11224	12397	11914	11121	11664	521.59	4.4%
Sample 3	42475	48901	43488	50585	46362	3451.40	7.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	715
Gene Name	C1R
UniProt ID	P00736

Applications	ELISA
Research Area	Complement,Immunology,Innate Immunity

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001458	EK1954	96 wells/kit	$499		Add to Cart

Human Complement C1R ELISA Kit PicoKine®

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