Human Collagen alpha-1(II) chain (COL2A1) ELISA kit

Human Collagen alpha-1(II) chain (COL2A1) ELISA kit

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Mfr.No.

CSB-EL005739HU

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

collagen, type II, alpha 1, ANFH, AOM, COL11A3, MGC131516, SEDC, cartilage collagen, chondrocalcin, collagen II, alpha-1 polypeptide, collagen, type II, alpha 1, ANFH, AOM, COL11A3, MGC131516, SEDC, cartilage collagen, chondrocalcin, collagen II, alpha-1 polypeptide

Recovery

The recovery of mouse NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-96
EDTA plasma (n=4)	98	95-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	88
	Range %	82-95
1:4	Average %	99
	Range %	94-103
1:8	Average %	102
	Range %	96-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nM BCE	OD1	OD2	Average	Corrected
200	2.402	2.435	2.419	2.259
100	1.702	1.789	1.746	1.586
50	0.966	1.026	0.996	0.836
25	0.575	0.596	0.586	0.426
12.5	0.349	0.364	0.357	0.197
6.25	0.288	0.305	0.297	0.137
3.12	0.209	0.201	0.205	0.045
0	0.165	0.155	0.160	?

Species

Homo sapiens

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P02458
Tissue Specificity	Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.

Applications	ELISA
Research Area	COL2A1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501850	CSB-EL005739HU	96T	$695	Add to Cart
BHE10501850	CSB-EL005739HU	5x96T	$3336	Add to Cart
BHE10501850	CSB-EL005739HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.