Matrices listed below were spiked with certain level of COL15A1 and the recovery rates were calculated by comparing the measured value to the expected amount of COL15A1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
94
EDTA plasma(n=5)
85-100
93
heparin plasma(n=5)
86-95
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL15A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
COL15A1 ELISA Kit allows for the in vitro quantitative determination of COL15A1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human COL15A1 (Collagen alpha-1 XV) ELISA Kit