Human CNDP1/Carnosine Dipeptidase 1 ELISA Kit PicoKine®

Mfr.No.

EK1957

Sensitivity

<50 pg/ml

Detection Range

6.25 ng/ml - 400 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	6.25	12.5	25	50	100	200	400
O.D.	0.119	0.163	0.204	0.274	0.460	0.748	1.260	2.088

Description

Human CNDP1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CNDP1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Beta-Ala-His dipeptidase; CNDP dipeptidase 1; Caosine dipeptidase 1; Glutamate carboxypeptidase-like protein 2; Serum caosinase; CNDP1; CN1; CPGL2; UNQ1915/PRO4380

Immunogen

Expression system for standard: NS0; Immunogen sequence: S27-H507

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CNDP1

Subcellular Localization

Secreted

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	5364	12789	25697	5641	12786	25595
Standard deviation	193.1	537.14	1104.97	310.26	690.44	1377.74
CV (%)	3.6%	4.2%	4.3%	5.5%	5.4%	5.3%

Protein Function

Preferential hydrolysis of the beta-Ala-|-His dipeptide (caosine), and also anserine, Xaa-|-His dipeptides and other dipeptides including homocaosine.

Background

Beta-Ala-His dipeptidase is an enzyme that in humans is encoded by the CNDP1 gene. It is mapped to 18q22.3. This gene encodes a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human caosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	5364	5541	5698	5319	5481	279.51	5.1%
Sample 2	12789	12898	14236	13621	13386	736.23	5.5%
Sample 3	25697	26546	26973	26459	26419	1241.68	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	84735
Gene Name	CNDP1
UniProt ID	Q96KN2
Tissue Specificity	Found in serum and adult nervous central system. Absent in serum from patients with homocaosinosis.

Applications	ELISA
Research Area	Cancer,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001457	EK1957	96 wells/kit	$499		Add to Cart

Human CNDP1/Carnosine Dipeptidase 1 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human CNDP1/Carnosine Dipeptidase 1 ELISA Kit PicoKine®

Rate it now..!!