Human CNDP1/Carnosine Dipeptidase 1 ELISA Kit PicoKine®

Mfr.No.

EK1957

Sensitivity

<50 pg/ml

Detection Range

6.25 ng/ml - 400 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	6.25	12.5	25	50	100	200	400
O.D.	0.119	0.163	0.204	0.274	0.460	0.748	1.260	2.088

Description

Human CNDP1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CNDP1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Beta-Ala-His dipeptidase; CNDP dipeptidase 1; Carnosine dipeptidase 1; Glutamate carboxypeptidase-like protein 2; Serum carnosinase; CNDP1; CN1; CPGL2; UNQ1915/PRO4380

Immunogen

Expression system for standard: NS0; Immunogen sequence: S27-H507

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CNDP1

Subcellular Localization

Secreted

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	5364	12789	25697	5641	12786	25595
Standard deviation	193.1	537.14	1104.97	310.26	690.44	1377.74
CV (%)	3.6%	4.2%	4.3%	5.5%	5.4%	5.3%

Protein Function

Preferential hydrolysis of the beta-Ala-|-His dipeptide (carnosine), and also anserine, Xaa-|-His dipeptides and other dipeptides including homocarnosine.

Background

Beta-Ala-His dipeptidase is an enzyme that in humans is encoded by the CNDP1 gene. It is mapped to 18q22.3. This gene encodes a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	5364	5541	5698	5319	5481	279.51	5.1%
Sample 2	12789	12898	14236	13621	13386	736.23	5.5%
Sample 3	25697	26546	26973	26459	26419	1241.68	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	84735
Antigen	CNDP1/Carnosine
Gene Name	CNDP1
UniProt ID	Q96KN2
Tissue Specificity	Found in serum and adult nervous central system. Absent in serum from patients with homocarnosinosis.

Applications	ELISA
Research Area	Cancer,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001457	EK1957	96 wells/kit	$499		Add to Cart

Human CNDP1/Carnosine Dipeptidase 1 ELISA Kit PicoKine®

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