Human CLEC10A ELISA Kit PicoKine®

Mfr.No.

EK2048

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.075	0.166	0.229	0.356	0.588	1.016	1.822	2.411

Description

Human CLEC10A ELISA Kit PicoKine® (96 Tests). Quantitate Human CLEC10A in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-type lectin domain family 10 member A; C-type lectin superfamily member 14; Macrophage lectin 2; CD301; CLEC10A; CLECSF13; CLECSF14; HML

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q61-H316

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CLEC10A

Subcellular Localization

Membrane. Single-pass type II membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	34	244	404	37	249	456
Standard deviation	1.87	12.4	17.38	2.26	16.4	34.2
CV (%)	5.5%	5.1%	4.3%	6.1%	6.6%	7.5%

Protein Function

Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells.

Background

C-type lectin domain family 10 member A also known as CLEC10A is a protein that in humans is encoded by the CLEC10A gene. This gene is mapped to 17p13.1. This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein tuover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	34	38	33	32	34	2.27	6.6%
Sample 2	244	232	243	247	241	5.67	2.3%
Sample 3	404	404	433	399	410	13.43	3.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	10462
Gene Name	CLEC10A
UniProt ID	Q8IUN9

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001560	EK2048	96 wells/kit	$750		Add to Cart

Human CLEC10A ELISA Kit PicoKine®

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