Human Cleavage and Polyadenylation Specific Factor 1

Mfr.No.

E2465Hu

Alternative Names

Cleavage and polyadenylation specificity factor 160 kDa subunit;Cleavage and polyadenylation specificity factor subunit 1;CPSF 1;CPSF 160 kDa subunit;CPSF1

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

160884

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

4.46ng/L

Background

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction (PubMed: 14749727). May play a role in eye morphogenesis and the development of retinal ganglion cell projections to the midbrain (By similarity).

Gene ID	29894
UniProt ID	Q10570
Antigen	CPSF1

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E2465Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.412	2.328	2.312
	2.243
600ng/L	1.256	1.307	1.292
	1.358
300ng/L	0.722	0.718	0.702
	0.713
150ng/L	0.395	0.391	0.376
	0.388
75ng/L	0.149	0.149	0.134
	0.15
0ng/L	0.017	0.016	0
	0.014

Precision

We measured random samples of E2465Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	797.3	23.44	2.9
Intra-Assay	2	18	285.3	15.79	5.5
Intra-Assay	3	18	489.6	26.95	5.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104003	E2465Hu	48T	$320		Add to Cart
BHE12104003	E2465Hu	96T	$458		Add to Cart

Human Cleavage and Polyadenylation Specific Factor 1 - CPSF1 ELISA Kit

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