Human Claudin 8 - CLDN8 ELISA Kit

Mfr.No.

E2306Hu

Sensitivity

0.018ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Claudin 8 (also known as CLDN8) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Claudin-8;CLDN8;HEL-S-79

Molecular Weight

24845

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2306Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.54	0.09	5.54
Intra-Assay	2	18	4.33	0.14	3.23
Intra-Assay	3	18	1.96	0.09	4.75

Background

Tight-junction protein required for paracellular chloride transport in the kidney. Mediates recruitment of CLDN4 to tight junction in the kidney. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Protocol

These standard curves of E2306Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.661	2.605	2.552
	2.548
4ng/ml	1.528	1.511	1.459
	1.494
2ng/ml	0.95	0.852	0.799
	0.754
1ng/ml	0.396	0.457	0.405
	0.519
0.5ng/ml	0.257	0.257	0.205
	0.257
0ng/ml	0.053	0.052	0
	0.052

Gene ID	9073
UniProt ID	P56748

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103845	E2306Hu	48T	$320		Add to Cart
BHE12103845	E2306Hu	96T	$458		Add to Cart

Human Claudin 8 - CLDN8 ELISA Kit

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