Human Chromatin Assembly Factor 1 Subunit B

Mfr.No.

E5048Hu

Sensitivity

10.05ng/L

Detection Range

20-3800ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Chromatin Assembly Factor 1 Subunit B (also known as CHAF1B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CAF-1 subunit B;CAF-I 60 kDa subunit;CAF-I p60;CHAF 1B;CHAF1B;Chromatin assembly factor 1 subunit B;Chromatin assembly factor I p60 subunit;M-phase phosphoprotein 7

Molecular Weight

61493

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5048Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	529	21.46	4.1
Intra-Assay	2	18	691.6	24.46	3.5
Intra-Assay	3	18	887.5	26.59	3

Background

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Protocol

These standard curves of E5048Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.267	2.172	2.115
	2.077
1000ng/L	1.535	1.48	1.424
	1.426
500ng/L	0.577	0.653	0.597
	0.729
250ng/L	0.318	0.335	0.279
	0.352
125ng/L	0.117	0.144	0.088
	0.172
0ng/L	0.058	0.056	0
	0.055

Gene ID	8208
UniProt ID	Q13112

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106411	E5048Hu	48T	$320		Add to Cart
BHE12106411	E5048Hu	96T	$458		Add to Cart

Human Chromatin Assembly Factor 1 Subunit B - CHAF1B ELISA Kit

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