Human CHRDL1 ELISA Kit PicoKine®

Mfr.No.

EK1953

Sensitivity

<50 pg/ml

Detection Range

93.6 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.088	0.134	0.176	0.265	0.402	0.702	1.263	2.099

Description

Human CHRDL1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CHRDL1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Chordin-like protein 1; Neuralin-1; Neurogenesin-1; Ventroptin; CHRDL1; NRLN1

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-C450

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CHRDL1

Subcellular Localization

Secreted

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	146	511	1796	201	618	2851
Standard deviation	7.59	27.08	100.58	13.07	41.41	196.72
CV (%)	5.2%	5.3%	5.6%	6.5%	6.7%	6.9%

Protein Function

Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development.

Background

Chordin-like 1 is a protein that in humans is encoded by the CHRDL1 gene. It is mapped to Xq23. This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alteatively spliced transcript variants encoding different isoforms have been described.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	146	138	126	133	135	7.29	5.4%
Sample 2	511	424	442	453	457	32.57	7.1%
Sample 3	1796	1945	1901	2041	1920	88.03	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	91851
Gene Name	CHRDL1
UniProt ID	Q9BU40
Tissue Specificity	Expressed in the developing coea and in the eye anterior segment in addition to the retina. Differentially expressed in the fetal brain. There is high expression in cerebellum and neocortex. Expressed in retinal pericytes.

Applications	ELISA
Research Area	Neural Stem Cells,Neurogenesis,Neurology Process,Neuroscience,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001459	EK1953	96 wells/kit	$499		Add to Cart

Human CHRDL1 ELISA Kit PicoKine®

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