Human CHL1/L1CAM-2 ELISA Kit PicoKine®

Mfr.No.

EK1576

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.018	0.169	0.232	0.306	0.502	0.853	1.462	2.093

Description

Human CHL1/L1CAM-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human CHL1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neural cell adhesion molecule L1-like protein;Close homolog of L1;Processed neural cell adhesion molecule L1-like protein;CHL1;CALL;

Molecular Weight

135071 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: I25-Q1096

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CHL1

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein . Soluble forms produced by cleavage/shedding also exist. .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	291	1965	5542	267	2117	5729
Standard deviation	22.11	141.48	343.6	21.09	192.64	435.4
CV (%)	7.6%	7.2%	6.2%	7.9%	9.1%	7.6%

Protein Function

Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of inteeurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane (By similarity). .

Background

Neural cell adhesion molecule L1-like protein also known as close homolog of L1 (CHL1) is a protein that in humans is encoded by the CHL1 gene. This protein is a member of the L1 gene family of neural cell adhesion molecules. By FISH, the CHL1 gene was mapped to chromosome 3p26.1. The protein however has dynamic localisation, meaning that it has not only multiple roles in the cell, but also various locations. This protein may also play a role in the growth of certain cancers.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	291	285	268	269	278	9.98	3.5%
Sample 2	1965	1964	2077	2085	2022	58.31	2.8%
Sample 3	5542	5306	5133	4837	5204	257.08	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	10752
Gene Name	CHL1
UniProt ID	O00533
Protein Name	Neural cell adhesion molecule L1-like protein
Tissue Specificity	Expressed in the fetal and adult brain as well as in Schwann cell culture. Also detected in adult peripheral tissues. .

Applications	ELISA
Research Area	Actin Binding Proteins,Actin, etc.,Cell Adhesion Proteins,Cytoskeleton,Cytoskeleton/ECM,ECM Proteins,Membrane Proteins,Microfilaments,Neurogenesis,Neurology Process,Neuroscience,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001154	EK1576	96 wells/kit	$750		Add to Cart

Human CHL1/L1CAM-2 ELISA Kit PicoKine®

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