Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®

Mfr.No.

EK1199

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	200000000	20000000	2000000	200000	20000
O.D.	0.009	0.093	0.182	0.358	0.701	1.156	1.822	2.355

Description

Human CFD/Adipsin ELISA Kit PicoKine® (96 Tests). Quantitate Human CFD in cell culture supeatants, cell lysates, serum, plasma(heparin , EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Complement factor D;3.4.21.46;Adipsin;C3 convertase activator;Properdin factor D;CFD;DF, PFD;

Molecular Weight

27033 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: I26-A253

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CFD

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	787	2378	90804	804	2193	8576
Standard deviation	44.07	149.81	436.56	49.84	144.73	471.68
CV (%)	6.6%%%	6.6%%	4.8%	6.2%	6.6%	5.5%

Protein Function

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alteate pathway. Its function is homologous to that of C1s in the classical pathway.

Background

Complement factor D (CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alteative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	787	751	817	732	771	32.75	4.2%
Sample 2	2378	2440	2261	2414	2373	68.44	2.8%
Sample 3	9095	10188	9364	8739	9346	534.02	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1675
Gene Name	CFD
UniProt ID	P00746
Protein Name	Complement factor D
Sequence	Belongs to the peptidase S1 family.

Applications	ELISA
Research Area	Mesenchymal Stem Cells,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000816	EK1199	96 wells/kit	$499		Add to Cart

Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®

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