Human Ceramide Kinase - CERK ELISA Kit

Mfr.No.

E4144hu

Description

This sandwich kit is for the accurate quantitative detection of human Ceramide Kinase (also known as CERK) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Acylsphingosine kinase;Ceramide kinase;CERK;hCERK;Lipid kinase 4;LK4

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

59977

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.13ng/L

Background

Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate (PubMed: 11956206, PubMed: 16269826, PubMed: 19168031). Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines (PubMed: 11956206, PubMed: 16269826, PubMed: 19168031). Shows a greater preference for D-erythro isomer of ceramides (PubMed: 16269826). Binds phosphoinositides (PubMed: 19168031).

Gene ID	64781
UniProt ID	Q8TCT0
Antigen	CERK

Research Area

Cell Biology

Protocol

These standard curves of E4144hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.292	2.235	2.208
	2.178
600ng/L	1.391	1.372	1.345
	1.352
300ng/L	0.859	0.742	0.716
	0.626
150ng/L	0.463	0.478	0.452
	0.493
75ng/L	0.205	0.193	0.167
	0.182
0ng/L	0.028	0.026	0
	0.025

Precision

We measured random samples of E4144hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	415.7	22.42	5.4
Intra-Assay	2	18	782.8	30.86	3.9
Intra-Assay	3	18	182	9.92	5.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105547	E4144hu	48T	$320		Add to Cart
BHE12105547	E4144hu	96T	$458		Add to Cart

Human Ceramide Kinase - CERK ELISA Kit

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