Human Centromere Protein K - CENPK ELISA Kit

Mfr.No.

E5030Hu

Alternative Names

CENPK;CENP-K;Centromere protein K;Interphase centromere complex protein 37;p33;Protein AF-5alpha

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

31655

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

6.95ng/L

Background

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.

Gene ID	64105
UniProt ID	Q9BS16
Antigen	CENPK

Research Area

Others

Protocol

These standard curves of E5030Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.111	2.081	2.029
	2.051
800ng/L	1.388	1.366	1.313
	1.343
400ng/L	0.861	0.91	0.858
	0.96
200ng/L	0.443	0.433	0.381
	0.423
100ng/L	0.294	0.282	0.23
	0.27
0ng/L	0.045	0.052	0
	0.059

Precision

We measured random samples of E5030Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	204.5	11.16	5.5
Intra-Assay	2	18	1185.7	55.87	4.7
Intra-Assay	3	18	509.1	31.39	6.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106393	E5030Hu	48T	$320		Add to Cart
BHE12106393	E5030Hu	96T	$458		Add to Cart

Human Centromere Protein K - CENPK ELISA Kit

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