Human Centromere Protein I - CENPI ELISA Kit

Mfr.No.

E2886Hu

Alternative Names

CENPI;CENP-I;Centromere protein I;Follicle-stimulating hormone primary response protein;FSH primary response protein 1;FSHPRH1;Interphase centromere complex protein 19;Leucine-rich primary response protein 1;LRPR1;

Reactivity

Human

Detection Range

20-6000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

86720

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

10.36ng/L

Background

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Required for the localization of CENPF, MAD1L1 and MAD2 (MAD2L1 or MAD2L2) to kinetochores. Involved in the response of gonadal tissues to follicle-stimulating hormone.

Gene ID	2491
UniProt ID	Q92674
Antigen	CENPI

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E2886Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.28	2.328	2.28
	2.375
1600ng/L	1.3	1.256	1.208
	1.212
800ng/L	0.727	0.792	0.745
	0.858
400ng/L	0.44	0.437	0.389
	0.433
200ng/L	0.25	0.264	0.217
	0.278
0ng/L	0.048	0.047	0
	0.047

Precision

We measured random samples of E2886Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1203.08	56.28	4.68
Intra-Assay	2	18	1567.36	53.54	3.42
Intra-Assay	3	18	614.71	38.32	6.23

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104423	E2886Hu	48T	$320		Add to Cart
BHE12104423	E2886Hu	96T	$458		Add to Cart

Human Centromere Protein I - CENPI ELISA Kit

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