Human Centromere Protein H - CENPH ELISA Kit

Mfr.No.

E2976Hu

Sensitivity

0.054ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CENPH;CENP-H;Centromere protein H;Interphase centromere complex protein 35

Molecular Weight

28481

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2976Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.03	0.4	5.67
Intra-Assay	2	18	9.9	0.25	2.52
Intra-Assay	3	18	3.72	0.21	5.59

Background

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.

Protocol

These standard curves of E2976Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.645	2.643	2.613
	2.64
12ng/ml	1.438	1.458	1.428
	1.478
6ng/ml	0.901	0.863	0.833
	0.825
3ng/ml	0.415	0.406	0.376
	0.396
1.5ng/ml	0.263	0.262	0.233
	0.261
0ng/ml	0.029	0.03	0
	0.03

Gene ID	64946
UniProt ID	Q9H3R5

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104513	E2976Hu	48T	$320		Add to Cart
BHE12104513	E2976Hu	96T	$458		Add to Cart

Human Centromere Protein H - CENPH ELISA Kit

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