Human Cell Migration-inducing and Hyaluronan-binding Protein

Mfr.No.

E5542Hu

Alternative Names

CCSP1;Cell migration-inducing and hyaluronan-binding protein;HYBID;KIAA1199;TMEM2L

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

152998

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.026ng/ml

Background

Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.

Gene ID	57214
UniProt ID	Q8WUJ3
Antigen	KIAA1199

Research Area

Others

Protocol

These standard curves of E5542Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.072	2.105	2.044
	2.138
6ng/ml	1.468	1.473	1.411
	1.477
3ng/ml	0.754	0.782	0.721
	0.811
1.5ng/ml	0.425	0.444	0.383
	0.464
0.75ng/ml	0.25	0.241	0.18
	0.233
0ng/ml	0.058	0.062	0
	0.065

Precision

We measured random samples of E5542Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.37	0.25	3.93
Intra-Assay	2	18	1.31	0.07	5.17
Intra-Assay	3	18	4.24	0.27	6.25

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106904	E5542Hu	48T	$320		Add to Cart
BHE12106904	E5542Hu	96T	$458		Add to Cart

Human Cell Migration-inducing and Hyaluronan-binding Protein - CEMIP ELISA Kit

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