Human CEACAM1/Cd66A ELISA Kit PicoKine®

Mfr.No.

EK1361

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	10,000000	10,00000	10,0000	10,000	20,000
O.D.	0.096	0.220	0.286	0.505	0.814	1.279	1.970	2.589

Description

Human CEACAM1/Cd66A ELISA Kit PicoKine® (96 Tests). Quantitate Human CEACAM1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Carcinoembryonic antigen-related cell adhesion molecule 1;Biliary glycoprotein 1;BGP-1;CD66a;CEACAM1;BGP, BGP1;

Molecular Weight

57560 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q35-G428

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CEACAM1

Subcellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	463	2835	9439	432	3010	9810
Standard deviation	32.41	150.25	453.07	35.42	207.69	529.74
CV (%)	7%	5.3%	4.8%	8.2%	6.9%	5.4%

Background

CEACAM1, also known as CD66a (Cluster of Differentiation 66a), is a human glycoprotein encoded by a gene. This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 is mapped to 19q13.2 in human. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	463	407	434	425	432	20.24	4.6%
Sample 2	2835	2793	2564	2668	2715	106.64	3.9%
Sample 3	9439	8061	8668	8372	8635	511.4	5.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	634
Gene Name	CEACAM1
UniProt ID	P13688
Protein Name	Carcinoembryonic antigen-related cell adhesion molecule 1
Sequence	Belongs to the immunoglobulin superfamily. CEA family.

Applications	ELISA
Research Area	Cancer,Cell Type Markers,Human Protein,Microbiology,Oncoproteins/Suppressors,Protein,Tags & Cell Markers,Tumor Antigens,Tumor Associated,Tumor Biomarkers,Tumor Immunology,Tumor Suppressors,Tumor-Associated Antigens

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000953	EK1361	96 wells/kit	$499		Add to Cart

Human CEACAM1/Cd66A ELISA Kit PicoKine®

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