Human CDSN ELISA Kit PicoKine®

Mfr.No.

EK2160

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1,000	2,000
O.D.	0.016	0.033	0.065	0.122	0.289	0.594	1.052	1.887

Description

Human CDSN ELISA Kit PicoKine® (96 Tests). Quantitate Human CDSN in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K33-P529

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CDSN

Sample Type

Cell culture supeatants, Serum, Plasma

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	42	155	697	45	157	722
Standard deviation	2.31	9.61	33.46	2.75	8.48	25.99
CV(%)	5.5%	6.2%	4.8%	6.1%	5.4%	3.6%

Background

Coeodesmosin is a protein that in humans is encoded by the CDSN gene. This gene encodes a protein found in coeodesmosomes, which localize to human epidermis and other coified squamous epithelia. The encoded protein undergoes a series of cleavages during coeocyte maturation. This gene is highly polymorphic in human populations, and variation has been associated with skin diseases such as psoriasis, hypotrichosis and peeling skin syndrome. The gene is located in the major histocompatibility complex (MHC) class I region on chromosome 6.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	42	62	52	48	51	1.84	3.6%
Sample 2	155	167	185	172	170	9.347	5.5%
Sample 3	697	702	754	784	734	35.98	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1041
Gene Name	CDSN
UniProt ID	Q15517

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001705	EK2160	96 wells/kit	$499		Add to Cart

Human CDSN ELISA Kit PicoKine®

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