Human CD97 PicoKine® Quick ELISA Kit

Mfr.No.

FEK1402

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.004	0.102	0.175	0.333	0.559	1.021	1.672	2.054

Description

Human CD97/ADGRE5 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ADGRE5 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

CD97 antigen;Leukocyte antigen CD97;CD97;CD97 antigen subunit alpha;CD97 antigen subunit beta;CD97;

Molecular Weight

91869 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-Q398

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD97

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	192	1210	5765	193	1108	6274
Standard deviation	12.09	62.92	253.66	13.12	77.56	288.6
CV (%)	7%	5.2%	4.4%	6.8%	7%	4.6%

Background

Cluster of differentiation 97 is a protein also known as BL-Ac (F2) encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence in situ hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	192	169	180	190	182	9.14	5%
Sample 2	1210	1172	1113	1111	1151	41.72	3.6%
Sample 3	5765	6054	5419	5224	5615	318.79	5.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	976
Gene Name	ADGRE5
UniProt ID	P48960
Protein Name	CD97 antigen

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001888	FEK1402	96 wells/kit	$499		Add to Cart

Human CD97 PicoKine® Quick ELISA Kit

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