Human CD70 antigen(CD70) ELISA kit

Human CD70 antigen(CD70) ELISA kit

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Mfr.No.

CSB-EL004954HU

Alternative Names

CD70 molecule, CD27L, CD27LG, TNFSF7, CD27 ligand, CD70 antigen, Ki-24 antigen, surface antigen CD70, tumor necrosis factor (ligand) superfamily, member 7, tumor necrosis factor ligand superfamily, member 7

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat AG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-104
EDTA plasma (n=4)	97	95-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat AG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	87-101
1:2	Average %	86
	Range %	80-92
1:4	Average %	93
	Range %	90-95
1:8	Average %	95
	Range %	92-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.690	2.699	2.695	2.502
200	1.885	1.899	1.892	1.699
100	1.162	1.198	1.180	0.987
50	0.798	0.760	0.779	0.586
25	0.581	0.535	0.558	0.365
12.5	0.388	0.376	0.382	0.189
6.25	0.301	0.321	0.311	0.118
0	0.191	0.195	0.193	?

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	P32970
Antigen	CD70 molecule

Applications

ELISA

Research Area

CD70

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501730	CSB-EL004954HU	96T	$695	Add to Cart
BHE10501730	CSB-EL004954HU	5x96T	$3336	Add to Cart
BHE10501730	CSB-EL004954HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.