Human CD5L/CT-2 ELISA Kit PicoKine®

Activate the sample

Cell culture supeatants: add activating reagent pro rate, i.e. add 20?l of Solution A into 40?l of sample, 10 min later, add 20?l of Solution B. PH7.0-7.6.

Serum: add activating reagent pro rate, i.e. add 20?l of Solution A into 40?l of sample, 10 min later, add 20?l of Solution B. PH7.0-7.6. Prior to the assay, the activated sample requires a 2000-fold dilution. Add 10?l of activated sample into 390?l wash buffer (1 : 40). And then add 4?l of above diluted reagent into 196?l sample diluent buffer ( 1 : 50).

Plasma(heparin, EDTA): add activating reagent pro rate, i.e. add 20?l of Solution A into 40?l of sample, 10 min later, add 20?l of Solution B. PH7.0-7.6. Prior to the assay, the activated sample requires a 4000-fold dilution. Add 10?l of activated sample into 790?l wash buffer (1 : 80). And then add 4?l of above diluted reagent into 196?l sample diluent buffer (1 : 50).Optimal dilutions should be determined by end users.

It is unnecessary to activate the recombinant CD5L.

Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.