Human CD40/TNFRSF5 ELISA Kit PicoKine®

Mfr.No.

EK0702

Sensitivity

<1 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.010	0.035	0.077	0.141	0.221	0.419	0.997	1.720

Description

Human CD40/TNFRSF5 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD40 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CDw40;CD40;CD40;TNFRSF5;

Molecular Weight

30619 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-R193

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD40

Subcellular Localization

Isoform I: Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Serum, Plasma, Saliva, Urine, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	32	167	443	32	177	404
Standard deviation	1.66	9.18	34.99	1.92	10.79	38.78
CV (%)	5.2%	5.5%	7.9%	6%	6.1%	9.6%

Protein Function

Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

Background

CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	32	30	36	34	33	2.23	6.7%
Sample 2	167	185	189	177	179	8.41	4.6%
Sample 3	443	505	507	519	493	29.64	6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	958
Antigen	CD40/TNFRSF5
Gene Name	CD40
UniProt ID	P25942
Protein Name	Tumor necrosis factor receptor superfamily member 5
Tissue Specificity	B-cells and in primary carcinomas.
Sequence	Contains 4 TNFR-Cys repeats.

Applications	ELISA
Research Area	Adaptive Immunity,Atherosclerosis,B Cells,Cardiovascular,Hematopoietic Progenitors,Immunology,Lymphoid,Myeloid,Stem Cells,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000576	EK0702	96 wells/kit	$499		Add to Cart

Human CD40/TNFRSF5 ELISA Kit PicoKine®

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