Human CD300C ELISA Kit PicoKine®

Mfr.No.

EK1981

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.029	0.081	0.144	0.254	0.417	0.842	1.327	2.159

Description

Human CD300C ELISA Kit PicoKine® (96 Tests). Quantitate Human CD300C in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

CMRF35-like molecule 6; CLM-6; CD300 antigen-like family member C; CMRF35-A; CMRF-35; Immunoglobulin superfamily member 16; IgSF16; CD300c; CMRF35; CMRF35A; CMRF35A1; IGSF16

Immunogen

Expression system for standard: NS0; Immunogen sequence: G21-R183

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD300C

Subcellular Localization

Cell membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	48	226	852	55	349	964
Standard deviation	2.98	15.14	38.34	3.25	24.78	41.45
CV (%)	6.2%	6.7%	4.5%	5.9%	7.1%	4.3%

Background

CMRF35-like molecule 6 (CLM-6) also known as CD300 antigen-like family member C (CD300c) is a protein that in humans is encoded by the CD300C gene. It is mapped to 17q25.1. The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	48	50	50	57	51	3.41	6.6%
Sample 2	226	258	264	265	253	15.95	6.3%
Sample 3	852	940	931	798	880	58.54	6.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	10871
Gene Name	CD300C
UniProt ID	Q08708
Tissue Specificity	Present on the surface of monocytes, neutrophils, a proportion of peripheral blood T- and B-lymphocytes and lymphocytic cell lines.

Applications	ELISA
Research Area	Adaptive Immunity,B Cells,Immunology,T Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001486	EK1981	96 wells/kit	$750		Add to Cart

Human CD300C ELISA Kit PicoKine®

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