Human CD300A ELISA Kit PicoKine®

Mfr.No.

EK1980

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.002	0.037	0.090	0.211	0.516	1.269	1.818	2.331

Description

Human CD300A ELISA Kit PicoKine® (96 Tests). Quantitate Human CD300A in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

CMRF35-like molecule 8; CLM-8; CD300 antigen-like family member A; CMRF-35-H9; CMRF35-H9; CMRF35-H; IRC1/IRC2; Immunoglobulin superfamily member 12; IgSF12; Inhibitory receptor protein 60; IRp60; NK inhibitory receptor; CD300a; CD300A; CMRF35H; IGSF12; HS

Immunogen

Expression system for standard: NS0; Immunogen sequence: L18-P180

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD300A

Subcellular Localization

Cell membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	362	1768	9786	405	1772	9892
Standard deviation	218.82	111.38	469.73	19.85	131.13	524.28
CV (%)	5.2%	6.3%	4.8%	4.9%	7.4%	5.3%

Protein Function

Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but no

Background

CD300A (Cluster of Differentiation 300A) is a human gene. It is mapped to 17q25.1. This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	362	424	405	365	389	26.39	6.7%
Sample 2	1768	1661	1621	1762	1703	63.62	3.7%
Sample 3	9786	8596	9892	9589	9465	513.78	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	11314
Gene Name	CD300A
UniProt ID	Q9UGN4
Tissue Specificity	Expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.

Applications	ELISA
Research Area	Cell Type Markers,Immunology,Innate Immunity,Macrophage/Inflammation,NK Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001489	EK1980	96 wells/kit	$750		Add to Cart

Human CD300A ELISA Kit PicoKine®

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