Human CD30/TNFRSF8 ELISA Kit PicoKine®

Mfr.No.

EK0569

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.039	0.173	0.281	0.472	0.784	1.236	1.732	2.010

Description

Human CD30/TNFRSF8 ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 8;CD30L receptor;Ki-1 antigen;Lymphocyte activation antigen CD30;CD30;TNFRSF8;CD30, D1S166E;

Molecular Weight

63747 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: F19-K379

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD30

Subcellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	321	1322	3947	305	1371	3830
Standard deviation	25.03	64.77	157.88	28.06	93.22	214.48
CV (%)	7.8%	4.9%	4%	9.2%	6.8%	5.6%

Protein Function

Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B.

Background

CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	321	324	313	319	319	4.02	1.2%
Sample 2	1322	1420	1463	1452	1414	55.55	3.9%
Sample 3	3947	3780	4218	4450	4098	256.04	6.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	943
Antigen	CD30/TNFRSF8
Gene Name	TNFRSF8
UniProt ID	P28908
Protein Name	Tumor necrosis factor receptor superfamily member 8
Sequence	Contains 6 TNFR-Cys repeats.

Applications	ELISA
Research Area	Cancer,Cell Type Markers,Cytokines,Immunology,Innate Immunity,Receptors,TNF Superfamily,Tumor Biomarkers,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001023	EK0569	96 wells/kit	$499		Add to Cart

Human CD30/TNFRSF8 ELISA Kit PicoKine®

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