Human CD244/2B4 ELISA Kit PicoKine®

Mfr.No.

EK0926

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.025	0.199	0.292	0.464	0.934	1.510	2.026	2.547

Description

Human CD244/2B4 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD244 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Natural killer cell receptor 2B4;NK cell activation-inducing ligand;NAIL;NK cell type I receptor protein 2B4;NKR2B4;h2B4;SLAM family member 4;SLAMF4;Signaling lymphocytic activation molecule 4;CD244;CD244;2B4;

Molecular Weight

41616 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: C22-R221

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD244

Subcellular Localization

Membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	28	81	453	30	112	469
Standard deviation	1.34	4.62	20.84	1.53	6.61	30.02
CV (%)	4.8%	5.7%	4.6%	5.1%	5.9%	6.4%

Protein Function

Modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family. .

Background

CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead,the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	28	27	30	28	28	1.08	3.8%
Sample 2	81	79	81	73	78	3.27	4.1%
Sample 3	453	479	435	456	455	15.64	3.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	51744
Gene Name	CD244
UniProt ID	Q9BZW8
Protein Name	Natural killer cell receptor 2B4
Tissue Specificity	Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils. .

Applications	ELISA
Research Area	Cell Type Markers,Hematopoietic Progenitors,Immunology,Lymphoid,NK Cells,Stem Cells,T Lymphocytic Lineage

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000701	EK0926	96 wells/kit	$499		Add to Cart

Human CD244/2B4 ELISA Kit PicoKine®

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