Human Cd226 Antigen - CD226 ELISA Kit

Mfr.No.

E4093hu

Description

This sandwich kit is for the accurate quantitative detection of human CD226 Antigen (also known as CD226) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD 226;CD antigen CD226;CD226;CD226 antigen;DNAM-1;DNAX accessory molecule 1

Reactivity

Human

Detection Range

0.5-100ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

38614

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.27ng/ml

Background

Involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell (PubMed: 8673704). Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding (PubMed: 26755705).

Gene ID	10666
UniProt ID	Q15762
Antigen	CD226

Research Area

Immunology

Protocol

These standard curves of E4093hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.158	2.119	2.082
	2.08
32ng/ml	0.984	1.033	0.996
	1.082
16ng/ml	0.541	0.571	0.534
	0.6
8ng/ml	0.294	0.278	0.242
	0.263
4ng/ml	0.124	0.106	0.069
	0.088
0ng/ml	0.027	0.037	0
	0.047

Precision

We measured random samples of E4093hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	23.7	1.02	4.3
Intra-Assay	2	18	53.3	1.65	3.1
Intra-Assay	3	18	10.2	0.54	5.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105500	E4093hu	48T	$320		Add to Cart
BHE12105500	E4093hu	96T	$458		Add to Cart

Human Cd226 Antigen - CD226 ELISA Kit

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