Human CCL4/MIP-1 Beta ELISA Kit PicoKine®

Mfr.No.

EK0450

Sensitivity

<1 pg/ml

Detection Range

7.8 pg/ml - 500 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.008	0.041	0.093	0.155	0.346	0.651	0.909	1.939

Description

Human CCL4/MIP-1 Beta ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine 4;G-26 T-lymphocyte-secreted protein;HC21;Lymphocyte activation gene 1 protein;LAG-1;MIP-1-beta (1-69);Macrophage inflammatory protein 1-beta;MIP-1-beta;PAT 744;Protein H400;SIS-gamma;Small-inducible cytokine A4;T-cell activation protein 2;ACT-2;MIP-1-beta (3-69);CCL4;LAG1, MIP1B, SCYA4;

Molecular Weight

10212 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-N92

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CCL4

Subcellular Localization

Secreted.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	13	90	254	12	95	256
Standard deviation	0.63	4.95	13.2	0.62	6.84	15.87
CV (%)	4.9%	5.5%	5.2%	5.2%	7.2%	6.2%

Protein Function

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta (3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. .

Background

Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	13	11	13	14	12	1.08	9%
Sample 2	90	88	90	93	90	1.78	1.9%
Sample 3	254	290	285	250	269	17.89	6.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6351
Antigen	CCL4/MIP-1
Gene Name	CCL4
UniProt ID	P13236
Protein Name	C-C motif chemokine 4
Sequence	Belongs to the intercrine beta (chemokine CC) family.

Applications	ELISA
Research Area	Antiviral Signaling,Chemokines,Immune System Diseases,Immunology,Innate Immunity,Macrophage/Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000446	EK0450	96 wells/kit	$499		Add to Cart

Human CCL4/MIP-1 Beta ELISA Kit PicoKine®

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