Human CCL24/Eotaxin-2 ELISA Kit PicoKine®

Mfr.No.

EK0331

Sensitivity

<2 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.023	0.051	0.151	0.222	0.484	0.859	1.441	1.979

Description

Human CCL24/Eotaxin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL24 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine 24;CK-beta-6;Eosinophil chemotactic protein 2;Eotaxin-2;Myeloid progenitor inhibitory factor 2;MPIF-2;Small-inducible cytokine A24;CCL24;MPIF2, SCYA24;

Molecular Weight

13134 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V27-C119

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CCL24

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	75	209	857	70	228	777
Standard deviation	3.67	13.16	44.56	4.13	16.41	41.18
CV (%)	4.9%	6.3%	5.2%	5.9%	7.2%	5.3%

Protein Function

Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.

Background

Chemokine (C-C motif) ligand 24 (CCL24) also known as myeloid progenitor inhibitory factor 2 (MPIF-2) or eosinophil chemotactic protein 2 (eotaxin-2) is a protein that in humans is encoded by the CCL24 gene. By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23. CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	75	82	86	80	80	3.96	4.9%
Sample 2	209	190	207	207	203	7.69	3.7%
Sample 3	857	1006	836	933	908	67.1	7.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6369
Gene Name	CCL24
UniProt ID	O00175
Protein Name	C-C motif chemokine 24
Tissue Specificity	Activated monocytes and activated T lymphocytes.
Sequence	Belongs to the intercrine beta (chemokine CC) family.

Applications	ELISA
Research Area	Chemokines,Immunology,Innate Immunity

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000358	EK0331	96 wells/kit	$499		Add to Cart

Human CCL24/Eotaxin-2 ELISA Kit PicoKine®

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