Human CCL19/MIP-3 Beta ELISA Kit PicoKine®

Mfr.No.

EK0456

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.030	0.066	0.128	0.191	0.395	0.770	1.506	2.265

Description

Human CCL19/MIP-3 Beta ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL19 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine ;CCL19 ;hCG_20476 ;

Molecular Weight

10993 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G22-S98

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CCL19

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	29	234	421	27	213	398
Standard deviation	1.39	16.7%7	26.94	1.35	16.4%7	32.23
CV (%)	4.8%	6.7%	6.4%	5%	7.5%	8.1%

Background

Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine?belonging to the CC chemokine family that is also known as?EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta? (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was map to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	29	30	31	29	29	0.82	2.8%
Sample 2	234	255	249	222	240	12.9	5.3%
Sample 3	421	456	425	465	441	19.07	4.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6363
Gene Name	CCL19
UniProt ID	Q6IBD6
Protein Name	C-C motif chemokine 19

Applications	ELISA
Research Area	Chemokines,G Protein Signaling,Immunology,Innate Immunity,Macrophage/Inflammation,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000451	EK0456	96 wells/kit	$499		Add to Cart

Human CCL19/MIP-3 Beta ELISA Kit PicoKine®

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