Human CCL18/PARC ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0686

Detection Range

7.8 pg/ml - 500 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.006	0.045	0.075	0.186	0.377	0.754	1.505	2.145

Description

Human CCL18/PARC ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CCL18 in cell culture supeatants and serum.

Alternative Names

C-C motif chemokine 18;Alteative macrophage activation-associated CC chemokine 1;AMAC-1;CC chemokine PARC;Dendritic cell chemokine 1;DC-CK1;Macrophage inflammatory protein 4;MIP-4;Pulmonary and activation-regulated chemokine;Small-inducible cytokine A18;CCL18 (1-68);CCL18 (3-69);CCL18 (4-69);CCL18;AMAC1, DCCK1, MIP4, PARC, SCYA18;

Molecular Weight

9849 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A21-A89

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CCL18/PARC

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	49	199	346	53	218	362
Standard deviation	1.62	7.36	14.88	3.66	15.7	27.87
CV (%)	3.3%	3.7%	4.3%	6.9%	7.2%	7.7%

Protein Function

Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. .

Background

Chemokine (C-C motif) ligand 18 (CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	6362
Gene Name	CCL18
UniProt ID	P55774
Protein Name	C-C motif chemokine 18
Tissue Specificity	Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid. .
Sequence	Belongs to the intercrine beta (chemokine CC) family.

Applications	ELISA
Research Area	Cancer,Chemokines,Immunology,Innate Immunity,Oncoproteins,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000009	EZ0686	5 plates/kit	$500		Add to Cart

Human CCL18/PARC ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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