Human CCL16/HCC-4 PicoKine® Quick ELISA Kit

Mfr.No.

FEK1127

Sensitivity

<10 pg/ml

Detection Range

7.8 pg/ml - 500 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.024	0.070	0.117	0.200	0.384	0.777	1.422	2.095

Description

Human CCL16/HCC-4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL16 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine 16;Chemokine CC-4;HCC-4;Chemokine LEC;IL-10-inducible chemokine;LCC-1;Liver-expressed chemokine;Lymphocyte and monocyte chemoattractant;LMC;Monotactin-1;MTN-1;NCC-4;Small-inducible cytokine A16;CCL16;ILINCK, NCC4, SCYA16;

Molecular Weight

13600 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-Q120

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CCL16

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	17	60	271	18	54	267
Standard deviation	0.85	3.18	20.86	1.02	3.56	21.36
CV (%)	5%	5.3%	7.7%	5.7%	6.6%	8%

Background

Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine (LEC) and Monotactin-1 (MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	17	16	17	17	16	0.43	2.6%
Sample 2	60	53	52	58	55	3.34	6%
Sample 3	271	227	243	230	242	17.38	7.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	6360
Gene Name	CCL16
UniProt ID	O15467
Protein Name	C-C motif chemokine 16

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001890	FEK1127	96 wells/kit	$499		Add to Cart

Human CCL16/HCC-4 PicoKine® Quick ELISA Kit

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