Human CCL15/Mip 1 Delta ELISA Kit PicoKine®

Mfr.No.

EK1120

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.042	0.092	0.121	0.194	0.319	0.570	1.023	2.010

Description

Human CCL15/Mip 1 Delta ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL15 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine 15;Chemokine CC-2;HCC-2;Leukotactin-1;LKN-1;MIP-1 delta;Macrophage inflammatory protein 5;MIP-5;Mrp-2b;NCC-3;Small-inducible cytokine A15;CCL15 (22-92);CCL15 (25-92);CCL15 (29-92);CCL15;MIP5, NCC3, SCYA15;

Molecular Weight

12248 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q22-I113

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CCL15

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	33	185	439	31	188	417
Standard deviation	2.31	9.8	31.6	2.75	13.34	37.11
CV (%)	7%	5.3%	7.2%	8.9%	7.1%	8.9%

Protein Function

Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15 (22-92), CCL15 (25-92) and CCL15 (29-92) are more potent chemoattractants than the small-inducible cytokine A15. .

Background

Chemokine (C-C motif) ligand 15 (CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP5 and HCC-2. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. The human CCL15 gene spans four exons and is located in a head-to-tail orientation on chromosome 17 with the gene of another CC chemokine known as CCL14.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	33	29	29	33	31	2	6.4%
Sample 2	185	185	181	184	183	1.63	0.8%
Sample 3	439	430	478	439	446	18.55	4.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6359
Gene Name	CCL15
UniProt ID	Q16663
Protein Name	C-C motif chemokine 15
Tissue Specificity	Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15 (22-92), CCL15 (25-92) and CCL15 (29-92) are found in high levels in synovial fluids from rheumatoid patients. .
Sequence	Belongs to the intercrine beta (chemokine CC) family.

Applications	ELISA
Research Area	Angiogenesis,Cardiovascular,Chemokines,Immunology,Innate Immunity,Macrophage/Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000774	EK1120	96 wells/kit	$499		Add to Cart

Human CCL15/Mip 1 Delta ELISA Kit PicoKine®

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