Human CCL14/HCC-1 ELISA Kit PicoKine®

Mfr.No.

EK1123

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentratio (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.007	0.023	0.069	0.161	0.359	0.670	1.288	1.955

Description

Human CCL14/HCC-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL14 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine 14;Chemokine CC-1/CC-3;HCC-1/HCC-3;HCC-1 (1-74);NCC-2;Small-inducible cytokine A14;HCC-1 (3-74);HCC-1 (4-74);HCC-1 (9-74);CCL14;NCC2, SCYA14;

Molecular Weight

10678 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T20-N93

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CCL14

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	37	238	445	40	227	446
Standard deviation	1.92	13.8	25.81	2.08	15.89	33.45
CV (%)	5.2%	5.8%	5.8%	5.2%	7%	7.5%

Protein Function

Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca (2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1 (9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. .

Background

Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	37	34	36	35	35	1.11	3.1%
Sample 2	238	250	251	216	238	14.09	5.9%
Sample 3	445	501	523	499	492	28.72	5.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6358
Gene Name	CCL14
UniProt ID	Q16627
Protein Name	C-C motif chemokine 14
Tissue Specificity	Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma. .
Sequence	Belongs to the intercrine beta (chemokine CC) family.

Applications	ELISA
Research Area	Chemokines,Cofactors, Vitamins/Minerals,Immunology,Innate Immunity,Metabolism,Pathways and Processes,Signal Transduction,Vitamins/Minerals

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000777	EK1123	96 wells/kit	$499		Add to Cart

Human CCL14/HCC-1 ELISA Kit PicoKine®

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