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Boster
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Boster
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| Mfr.No. | EZ1123 | ||||||||||||||||||||||||||||||||||||
| Detection Range | 15.6 pg/ml - 1,000 pg/ml | ||||||||||||||||||||||||||||||||||||
| Reactivity | Human | ||||||||||||||||||||||||||||||||||||
| Sample Volume |
Description |
Human CCL14 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CCL14 in cell culture supeatants, serum and plasma (heparin, EDTA).
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Immunogen |
Expression system for standard: E.coli; Immunogen sequence: T20-N93
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Kit Components |
Description |
Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins.
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Specificity |
Natural and recombinant human CCL14
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Sample Type |
Cell culture supeatants, Serum, Plasma
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Anticoagulant |
heparin or EDTA
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Background |
Chemokine(C-C motif) ligand 14(CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.
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Notes |
Material Required But Not Provided: |
1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered. 10. Substrate Solution: Tetramethylbenzidine 11. Stop Solution: 2 N H2SO4 12. Wash Buffer . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 12 are included in the EZ Set Accessory Kit 
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