Human Cathepsin L ELISA Kit PicoKine®

Mfr.No.

EK0676

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.118	0.175	0.210	0.291	0.478	0.821	1.458	2.095

Description

Human Cathepsin L ELISA Kit PicoKine® (96 Tests). Quantitate Human CTSL in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cathepsin L1;3.4.22.15;Cathepsin L;Major excreted protein;MEP;Cathepsin L1 heavy chain;Cathepsin L1 light chain;CTSL;CTSL1;

Molecular Weight

37564 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E113-V333

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Cathepsin L

Subcellular Localization

Lysosome.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	204	726	1367	205	706	1305
Standard deviation	10.2	29.76	66.98	11.07	36.71	82.21
CV (%)	5%	4.1%	4.9%	5.4%	5.2%	6.3%

Protein Function

Important for the overall degradation of proteins in lysosomes.

Background

Cathepsin L is an important lysosomal endopeptidase enzyme which is involved in the initiation of protein degradation. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. The gene is mapped to 9q21-q22. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein tuover, antigen and proprotein processing, and apoptosis. Cathepsin L has been reported in many organisms including fish, birds and mammals.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	204	202	210	182	199	10.52	5.2%
Sample 2	726	748	779	754	751	18.87	2.5%
Sample 3	1367	1597	1607	1555	1531	96.95	6.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1514
Gene Name	CTSL
UniProt ID	P07711
Protein Name	Cathepsin L1

Applications	ELISA
Research Area	Cell Adhesion Proteins,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Membrane Proteins,Neuroscience,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001021	EK0676	96 wells/kit	$499		Add to Cart

Human Cathepsin L ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Cathepsin L ELISA Kit PicoKine®

Rate it now..!!